Trophozoite of E. histolytica in sygmoidoscopied material with prominent ingested red blood cells (H & E stain).
Uninucleate and binucleate cysts of E. histolytica in iodine preparation
Histolytica exists in two forms – trophozoite and cyst .
Trophozoites are motile by means of short and blunt pseudopodia hence the outline is not clearly defmed. A clear ectoplasm and a dense granular endoplasm are well appreciated. The dense endoplasm contains nucleus, food vacuoles (sometimes host red blood cells) .
Cysts, on the other hand, are round and resistant to external environment. It may contain one to four nuclei depending on the stage of maturation.
E. histolytica causes amoebic dysentery. Occasionally, it also causes abscess in liver, Iung and other parts of the body including the brain.
Laboratory diagnosis of intestinal amoebiasis is usually done by the examination of stool samples using various techniques, most common being saline and iodine preparation. Trophozoites in faecal samples, however, are seen only in dysentric stool.
Dr.B.Z
reference :ATLAS OF MEDICAL PARASITOLOGY ,Shiba Kumar Rai 😀
