Triple Sugar Iron Agar
Triple Sugar Iron medium is a differential medium that can distinguish between a number of Gram-negative enteric bacteria based on their physiological ability (or lack thereof) to:
a. ...
Coagulase Test
Staphylococcus aureus is known to produce coagulase, which can clot plama into gel in tube or agglutinate cocci in slide. This test is useful in differentiating S.aureus from other ...
Catalase Test
The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative).
The enzyme, catalase, is produced by bacteria that respire using ...
Citrate utilization Test
Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Simmons citrate agar contains sodium citrate as the sole source of carbon, ammonium dihydrogen ...
Urease Test
Urease test principle
Many organisms especially those that infect the urinary tract, have a urease enzyme which is able to split urea in the presence of water to release ammonia and ...
Swarming growth on Blood Agar
Swarming or spreading in wave form is seen with motile organisms particularly Proteus mirabilis.
Cause of swarming – The flagella of bacteria are naturally set to spin counter-clockwise ...
Microscopic Morphologic Features of the Yeast Forms of Various Dimorphic Fungi
The yeast form of Blastomyces dermatitidis is a large, 10-15 µm in diameter, thick-walled yeast cell that characteristically produces a single bud attached by a broad base.
Coccidioides ...
Microscopic Morphologic Features of the Mold Forms of Various Dimorphic Fungi
The spiked (echinulate) macroconidia seen in Image below is characteristic of the mold form of Histoplasma capsulatum.
The mold form of Coccidioides immitis (Image below) is characterized ...
GasPak System (The Anaerobic jar)
Agar plates are inoculated and placed in the jar. Water is added to the GasPak generator envelope, causing the evolution of H2 and CO2. he hydrogen reacts with oxygen on the surface ...
Nagler’s reaction (Lecithinase test)
Nagler’s reaction is a test for the identification of alpha toxin of Clostridium perfringens. The toxin is lecithinase which hydrolyses the phospholipid lecithin (a component ...
Media used for Cultivation of Anaerobic Bacteria
Cooked Meat Broth (CMB) is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. The medium can be inoculated with ...
Most Common Species of Clostridium
Clostridium consists of around 100 species that include common free-living bacteria as well as important pathogens There are five main species responsible for disease in humans.
C. ...
