Nagler’s reaction (Lecithinase test)

Nagler’s reaction is a test for the identification of alpha toxin of Clostridium perfringens. The toxin is lecithinase which hydrolyses the phospholipid lecithin (a component ...

Media used for Cultivation of Anaerobic Bacteria

Cooked Meat Broth (CMB) is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. The medium can be inoculated with ...

Most Common Species of Clostridium

Clostridium consists of around 100 species that include common free-living bacteria as well as important pathogens There are five main species responsible for disease in humans. C. ...

Clostridium Characteristics

Clostridium are Gram-positive obligate anaerobes bacteria capable of producing endospores which protect them in harmful environment .  Individual cells are rod shaped while the spores ...

Serratia marcescens colonies features on 30c incubation

Serratia marcescens. These gram negative rods produce mucoid colonies which have entire margins and umbonate elevation. Note that there are both red and white colonies present on ...

Desoxycholate Citrate Agar (DCA)

Desoxycholate Citrate Agar is ideal for the investigation of pathogenic enterobacteria. The gram-positive organisms are totally inhibited by the sodium citrate and sodium desoxycholate. ...

Salmonellae growing on XLD Agar

XLD agar is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples. Salmonellae metabolise thiosulfate to produce hydrogen sulfide, ...

Quellung test or Capsular swelling

Quellung Test is an increase in the opacity and visibility of the capsule of encapsulated organisms resulting from exposure to specific, agglutinating, anticapsular antibodies. This ...

Inulin fermentation test for penumococci differentiation

Inulin Fermentation Test: this test is useful to differentiate Pneumococci from Streptcocci as the latter do not ferment Inulin sugar while Pneumococci do ferment it.

mycobacterium tuberclosis cording

Mycobacterium tuberculosis to grow end to end giving them a rope or cord-like appearance when viewed on a stained slide through a microscope.

How to quantitatively report Acid Fast Bacilli in stained smear

If definite Acid Fast Bacilli are seen, report it as AFB positive. But, it is better to report the result quantitatively as follows: > 10 AFB/high power field                ...

Gram stain for sputum smear from cystic fibrosis patient

This is gram stain of sputum specimen taken from patient with cystic fibrosis: notice the white blood cells and the encapsulated gram negative rods.
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