Cooked Meat Broth (CMB) is recommended for the cultivation of aerobic, microaerophilic, and anaerobic microorganisms, especially Clostridium species. The medium can be inoculated with a pure culture of an isolated colony, macerated tissue or liquid from a clinical specimen by heavily inoculate in the area of meat particles then Incubate the tubes with caps tightened at 35 degrees C. for up to 7 days. Growth or turbidity should be confirmed by gram stain and subcultured onto an appropriate plated growth medium. CMB contain unsaturated fatty acids which take up oxygen.
Cooked Meat Broth (CMB) for Anaerobic Bacteria
Cooked Meat Broth (CMB)
Thioglycollate Broth is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, specially used for sterility tests and the cultivation of microaerophilic and anaerobic organisms. it contains sodium thioglycollate, which reacts with molecular oxygen keeping free oxygen levels low. The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.
Thioglycollate Broth
