- Cysts of Entamoeba hartmanni are similar to those of E. histolytica but smaller, measuring 5-10 µm.
- Mature cysts contain four nuclei that possess a small, discrete centrally-located karyosome and evenly-distributed peripheral chromatin.
- Cysts may not be visible in unstained specimens.
- The cytoplasm in mature cysts may contain diffuse glycogen and rounded or elongated chromatoid bodies with rounded ends.