Fluorescence and Fluorochromes staining techniques overview

Fluorochromes are certain dyes that give off light when excited, and this is called Fluorescence and the color of light depends on the dye and the filters used with the fluorescent microscopy.

Staining Techniques : Fluorochroming: by direct chemical interaction between the dye used and the stained organisms.

  • Acridine orange: stains nucleic acid and it’s useful for cell-wall deficient organisms.
Cytomegalovirus-infected cells (acridine orange)

Cytomegalovirus-infected cells (acridine orange)

  • Auramine-rhodamine: bind to mycolic acids in nearly all Mycobacteria.
Auramine-rhodamine stain on mycobacterium

Auramine-rhodamine stain on mycobacterium

  • Calcofour white: binds to chitin in cell walls of fungi.
Calcoflour white - Mould Staining

Calcoflour white – Mould Staining

Yeast staining using Calcoflour white

Yeast staining using Calcoflour white

  • Immunofluorescence: fluorochrome is bound to an antibody that binds specifically to certain antigen

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