Fluorochromes are certain dyes that give off light when excited, and this is called Fluorescence and the color of light depends on the dye and the filters used with the fluorescent microscopy.
Staining Techniques : Fluorochroming: by direct chemical interaction between the dye used and the stained organisms.
- Acridine orange: stains nucleic acid and it’s useful for cell-wall deficient organisms.
- Auramine-rhodamine: bind to mycolic acids in nearly all Mycobacteria.
- Calcofour white: binds to chitin in cell walls of fungi.
- Immunofluorescence: fluorochrome is bound to an antibody that binds specifically to certain antigen