Agar plates are inoculated and placed in the jar. Water is added to the GasPak generator envelope, causing the evolution of H2 and CO2. he hydrogen reacts with oxygen on the surface of the palladium catalyst, forming water and establishing anaerobic conditions. Carbon dioxide aids growth of the anaerobes. An anaerobic indicator strip (a pad saturated with methylene blue solution) changes from blue to colorless in the absence of oxygen.

Picture of the anaerobic jar with inoculated Petri plaes, the GasPak generator envelope, and the anaerobic indicator strip.

GasPak system (Anaerobic jar)

GasPak generator envelope

GasPak system – notice the methylene blue indicator strip
Tags: anaerobes,
Anaerobic Bacteria,
anaerobic conditions,
Anaerobic jar,
Co2 generator,
GasPak,
GasPak envelope,
methylene blue,
palladium,
palladium catalyst,
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