Malachite green stain

Place the slide over a beaker of boiling water When there is condensation under the slide, flood with 5% solution of Malachite green stain 1min Wash in tap water Add 0.5% safranine ...

Chocolate agar (CHOC) or chocolate blood agar (CBA)

Chocolate agar is a non-selective, enriched growth medium. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C. Chocolate ...

Division patterns among cocci

 

Neisseria gonorrhoeae in electron microscope

  Neisseria gonorrhoeae diplococcus by electron microscope , notice capsule and many pili on surface , those pili enable the bacteria to adhere to human cells.

Streaking pattern of urine specimen

 

Using KOH Test as Gram stain confirmation

  the KOH test is a confirmation test of the Gram stain, especially with difficult bacterial species. The Gram stain is the basis for identification & classification in ...

E. coli and a non-lactose fermentation

MacConkey agar inoculated with lactose-fermenting Escherichia coli (at left) and non-lactose-fermenting Proteus (at right). Lactose-fermenting bacteria appear bright pink, while non-lactose-fermenting ...

Streptococcus Classification based on blood hemolysis

 

Neisseria meningitidis

Neisseria meningitidis  often referred to as meningococcus, Capsulated Gram-negative diplococcus aerobic Non motile nonspore forming catalase positive oxidase positive Acid production ...

ONPG Test – ( o-nitrophenyl-β-D-galactoside)

Purpose: this test is used to demonstrate the presence or absence of the enzyme B-galactosidase using the substrate Ortho-nitrophenyl-D-galactopyranoside in order to differentiate ...

Simmons Citrate Agar

Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Simmons citrate agar contains sodium citrate as the sole source of carbon, ammonium dihydrogen ...

Urease Test

Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide. The broth ...
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