Malachite green stain
Place the slide over a beaker of boiling water
When there is condensation under the slide, flood with 5% solution of Malachite green stain 1min
Wash in tap water
Add 0.5% safranine ...
Chocolate agar (CHOC) or chocolate blood agar (CBA)
Chocolate agar is a non-selective, enriched growth medium.
It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C.
Chocolate ...
Neisseria gonorrhoeae in electron microscope
Neisseria gonorrhoeae diplococcus by electron microscope , notice capsule and many pili on surface , those pili enable the bacteria to adhere to human cells.
Using KOH Test as Gram stain confirmation
the KOH test is a confirmation test of the Gram stain, especially with difficult bacterial species.
The Gram stain is the basis for identification & classification in ...
E. coli and a non-lactose fermentation
MacConkey agar inoculated with lactose-fermenting Escherichia coli (at left) and non-lactose-fermenting Proteus (at right). Lactose-fermenting bacteria appear bright pink, while non-lactose-fermenting ...
Neisseria meningitidis
Neisseria meningitidis often referred to as meningococcus, Capsulated Gram-negative diplococcus
aerobic
Non motile
nonspore forming
catalase positive
oxidase positive
Acid production ...
ONPG Test – ( o-nitrophenyl-β-D-galactoside)
Purpose:
this test is used to demonstrate the presence or absence of the enzyme B-galactosidase using the substrate Ortho-nitrophenyl-D-galactopyranoside in order to differentiate ...
Simmons Citrate Agar
Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Simmons citrate agar contains sodium citrate as the sole source of carbon, ammonium dihydrogen ...
Urease Test
Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide.
The broth ...



