Streptococcus-Optochin Sensitivity Testing
Optochin (ethylhydrocupreine hydrochloride), a quinine derivative, has a detergent-like action and causes selective lysis of pneumococci.
A sterile disk impregnated with optochin ...
Gram staining
Gram staining is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative).
Gram staining differentiates bacteria by the chemical ...
E-coli on the EMB plate
E.coli which is growing on the EMB plate below shows the green metallic shine that you sometimes see when cells release a lot of acid during lactose fermentation.
Staphylococcus epidermidis
-Gram-positive
-nonhemolytic on blood agar.
– nonmotile
-catalase :positive
-coagulase :negative
-facultative anaerobe
-oxidase : negative
– sensitive to novobiocin
– ...
Staphylococcus aureus
– gram positive cocci
– nonmotile
– Catalase: positive
– Oxidase: negative
– facultative anaerobic
– grape-like clusters under microscope
– ...
Haemophilus influenzae
– GRAM-NEGATIVE RODS
– NON-MOTILE
– NON-SPOREFORMING
– CATALASE: POSITIVE
– OXIDASE : POSITIVE
– FACULTATIVE ANAEROBIC
BASIC TESTS FOR IDENTIFICATION:
requirement ...
Antibiotic Sensitivity Test by Kirby-Bauer method
The Kirby-Bauer disk diffusion test is a standardized protocol for determining antibiotic susceptibility.
A pure culture of the test organism is inoculated on Mueller-Hinton agar.
Paper ...
