difference between Plasma and Serum

  • Plasma is the supernatant obtained after centrifugation of blood collected into a test tube containing anticoagulant to prevent clotting
  • Several anticoagulants are used in laboratory practice, the most common being lithium heparin and ethylenediaminetetraacetic acid (EDTA).
  • Heparin prevents clotting by binding to thrombin.
  • EDTA and citrate bind Ca2+ and Mg2+, thus interfering with the action of calcium and magnesium-dependent enzymes involved in the clotting cascade.
  • Citrate is used as an anticoagulant when blood is collected for transfusion.
  • Serum, on the other hand, is the supernatant obtained after a blood sample has been allowed to clot spontaneously (this usually requires 30–45 minutes). During clotting, fibrinogen is converted to fibrin as a result of proteolytic cleavage by thrombin, and so a major difference between plasma and serum is the absence of fibrinogen in serum.
Plasma and Serum Comparision

Plasma and Serum Comparision

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