X and V Factor Disks for the differentiation of Haemophilus species

X and V Factor Disks are paper disks impregnated with X (hemin) and V (nicotinamide adenine dinucleotide – NAD) growth factors. They are used for the differentiation of Haemophilus species.

X and V Factor Disks

X and V Factor Disks

Haemophilus influenzae require both X and V factor to grow while Haemophilus parainfluenzae requires V factor only for grow. Haemophilus ducreyi requires only X factor without need of V factor. We usually use Mueller Hinton Agar for the disk test.

Haemophilus influenzae growth around X - V Factor Disk

Haemophilus influenzae growth around X – V Factors Disk

Here is a table for Haemophilus species growth Around X – and V-Factor Disks:

Haemophilus species growth dependent on X and V Factor

Haemophilus species growth dependent on X and V Factor

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